The F2000 was seen used by a Militiaman in Takedown, pickups from downed police in No Russian with a Red Dot Sight and Scope, and is a pickup in Estate Takedown and The Only Easy Day...Was Yesterday. It is unlocked at Level 60 in Modern Warfare 2's multiplayer mode. The EGLM grenade launcher computer system isn't usable, although the regular leaf-sighted grenade launcher is. In singleplayer, it has two different types of Red Dot Sights. One version of the weapon, the F2000 Scoped, uses a Red Dot Sight whose appearance is based on the standard F2000 telescopic sight. The second, the F2000 Red Dot Sight, uses the Red Dot Sight seen on most of the other weapons. The version seen in game seems to be based on the F2000 Tactical, with MIL-STD Picatinny Rails on the top replacing the EGLM. Also, the Red Dot for this gun will inexplicably function during an EMP, where all other electronic sights fail.

In campaign mode, the F2000 is fairly similar to other assault rifles. In multiplayer however, its high vertical recoil makes it hard to use at mid to long range. Using short bursts can mitigate this somewhat and with Stopping Power is usually enough to take down an enemy. It is one of the more unused weapons due to its high unlock level and recoil. Despite the recoil, a player can keep almost on target at medium-close range, even on full auto. The F2000 has the highest rate of fire of all of the assault rifles. It has the third highest damage output per second out of all the assault rifles, due to its very high rate of fire, but it is beaten in just about every conceivable way by the TAR-21, which has the same magazine capacity, much less recoil, a much higher damage output due to its high damage per bullet, and a lower rate of fire which means that its magazine lasts longer.

Its inaccuracy can make it difficult to get headshots intentionally, though the very slight upward recoil will usually yield accidental headshots if aiming for the chest or neck.

Due to its rate of fire, the F2000 burns through magazine and ammunition reserves. In common usage, a full auto killing burst will use slightly less than half a magazine even with Stopping Power simply because of its high rate of fire. Players usually use Sleight of Hand or Scavenger to mitigate these problems.

Trivia

• The Red Dot Sight (in the form of the standard F2000 scope) is considered to be little help in multiplayer, as it obstructs your view.
• In real life, the standard F2000 scope is not an unmagnified red dot, but rather a magnified optic with a power of 1.6x.
• The Veteran II Challenge for the F2000 is different from every other Veteran II, due to not only the gun name changing but adding the manufacturer's name to the emblem (instead of just F2000 it is FN 2000).
• In real life, to reload the F2000, you must cycle the bolt. In the game, there is a bolt lock mechanism (which does not exist on the real F2000) that the player slaps during an empty reload.
• With the Silencer, the recoil becomes uncontrollable for long range shooting, relegating its use to close quarters combat.
• The F2000 mounts its own grenade launcher module, the GL1 EGLM in real life. It does not mount the M203, instead using the proprietary GL1 grenade launcher module developed especially for the F2000.
• In real life, while firing the weapon all the cases are ejected forward at the right side of the muzzle. In the game, they are ejected to the right, though still at the muzzle of the gun.
• The FN company used the P90 as a base for the F2000.
• The F2000 is one of the least used weapons in multiplayer due to its high ammo consumption, low damage, obstructive unique Red Dot Sight, high level unlock and large amount of recoil.
• When the enemy team in multiplayer activates a EMP, The F2000's Red Dot Sight will still work.
• The F2000's iron sights look very similar to those of the Lee-Enfield bolt-action rifle.